Research Focus
The Opavsky lab investigates the roles of DNA methyltransferases (DNMTs) in normal and malignant hematopoiesis, with a particular focus on how abnormal DNA methylation contributes to hematologic malignancies. Using mouse models, genome-wide analyses, and in vivo functional studies, the lab examines the tumor suppressor functions of DNMT3A and DNMT3B, as well as the impact of promoter hypomethylation on gene expression and cellular transformation, aiming to identify potential oncogenes for therapeutic targeting.
Responsibilities
In my research role, I utilized advanced laboratory techniques, including plasmid isolation and cell passaging, to support ongoing experiments. I analyzed the mechanisms by which shRNA mediates gene knockdown in T-cell lymphomas, gaining a deeper understanding of genetic regulation in cancer cells. Additionally, I collaborated closely with other members of the research team to ensure experiments were conducted efficiently and successfully, contributing to the overall progress of the laboratory’s projects.